SesameFG: Sesame Functional Genomics Database

 

 

To help the users who want to get quickly acquainted with the database and using the database for gene cloning on the genes of important agronomic traits, a step-by-step tutorial for the gene identification and cloning of seed coat color in sesame is available.

 

Step 1 – Population SNPs download

Step 2 – Phenotype query

 

Step 3 – GWAS (Wei et al., Nature Communications, 2015, 6, 8609)

Step 4 – Germplasm selection and population generation

Step 5 – SSRs query (from LG4 11007534 bp to 12007534 bp)

Step 6 – QTL mapping (Wei et al., Frontiers in Plant Science, 2016, 7, 1169)

Step 7 – Gene annotated with Literature query

Step 8 – Gene variation identified by BLAST (Gene sequence alignment of Zhongzhi13 and Mishuozhima)

Step 9 – Gene expression query (Sin_1016759)

Step 10 – Function validation of candidate genes (Wei et al., Nature Communications, 2015, 6, 8609)
Figure 12S c pin.tif